Effect of amino acid mixtures on nasal allergic responses induced by toluene diisocyanate in mice
Tzu-Hsiu Chena, Keiko Takeshitab, Kentaro Sakaia Tatsushi Komatsua, Yasuo Kishinoa, and Shigeru Yamamotoa

aDepartment of Nutrition, The University of Tokushima School of Medicine, Tokushima Japan;and bDepartment of Faculty of Environmental and Symbiotic Science;Prefectural University of Kumamoto, Kumamoto, Japan

Abstract:We studied the effect of various amino acid mixtures on nasal allergy induced by the intranasal application of toluene diisocyanate (TDI) in mice. In Experiment 1 (Exp. 1), mice were fed a 25% casein, soy protein isolate (SPI), egg white protein (EW) or gluten diet. In Experiment 2 (Exp. 2), mice were fed a 25% amino acid mixture diets patterned after casein (AA-casein), SPI (AA-SPI), EW protein (AA-EW) or gluten (AA-gluten). In Experiment 3 (Exp. 3) we modified the glutamine/glutamic acid (Gln/Glu) concentrations in the amino acid mixtures. Mice were fed a 25% AA-SPI, low Gln/Glu AA-SPI (LG-AA-SPI), AA-EW or high Gln/Glu AA-EW (HG-AA-EW) diet. At the 5th week, mice were divided into sensitized (sen-) and non-sensitized (ns-) groups. The mice in sensitized groups were treated with two courses of intranasal application of toluene diisocyanate (TDI) in ethyl acetate for 5 consecutive days, separated by 9 days rest. The non-sensitized groups of mice were treated with a vehicle. Nine days after the second sensitization, all mice were provoked by TDI. Nasal responses and serum IgE concentration were studied. The findings of Exp. 1 showed that the sen-EW group exhibited a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. The findings of Exp. 2 showed that the sen-EW group had a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. In Exp. 3, the AA-EW group showed a higher total nasal score and IgE concentration than the HG-AA-EW group, however, the findings of LG-AA-SPI and AA-SPI were similar. These findings demonstrated that amino acid mixtures affect nasal allergy induced by the intranasal application of TDI in mice. J. Med. Invest. 47:128-137, 2000

Keywords:allergic rhinitis, protein, amino acid, mouse

In previous studies we demonstrated that nucleosides and nucleotides enhance immunity (1, 2), however, they also aggravate nasal allergy caused by the intranasal administration of 2, 4-toluene diisocyanate (TDI) (3) and inflammatory bowel disease due to colitis by the administration of 2, 4, 6-trinitrobenzene sulphonic acid (TNBS) (4, 5). Arginine upregulates immunity (6), however, it also induces inflammatory bowel disease due to colitis by the administration of TNBS (7, 8). Glutamine (Gln) enhances immunity (9, 10) and alleviates inflammatory bowel disease induced by TNBS in rats (11), however, high supplementation aggravates the disease (12). Nucleosides, nucleotides, arginine and Gln do not possess antigenicity. From such background information, it was suggested that some amino acids or amino acid mixtures easily induce allergy.
In Exp. 1, we compared whether the 4 proteins had different effects on nasal allergy induced by TDI. In Exp. 2, we studied whether amino acid mixtures patterned after the proteins used in Exp. 1 had similar effects as the proteins. In Exp. 3, we changed the Gln and glutamic acid (Glu) concentrations to see the effect of these amino acids on nasal allergy induced by TDI. When we prepared the amino acid mixtures of the proteins, we used an amino acid composition table. Although the intact proteins contained Gln and Glu, in the table only Glu concentrations are shown. This is because Gln becomes Glu during the hydrolysis of protein for the measurement of amino acids. Gln has a strong influence on immunity (9, 10), therefore, in this study we used both Gln and Glu. Because the Gln concentration of soybean protein isolate (SPI) is high and that of egg white (EW) is low, in Exp. 3, we changed the Gln/Glu compositions of amino acid mixtures patterned after soybean protein isolate (AA-SPI) and egg white (AA-EW) to examine the effect of Gln/Glu on allergy.
Mice were administered a TDI solution into the nostrils to induce allergy. TDI is a polyurethane material than induces asthma among people working in polyurethane factories (13, 14). TDI is a very reactive chemical and easily combines with various proteins in the body, which become antigens. By the nasal administration of TDI, a nasal allergy model was produced in guinea pigs (15). We induced the nasal allergy model in mice using this method (3, 16) and studied the effects of proteins and amino acids on the allergy in mice.

MATERIALS AND METHODS
Animals and diets
Four week old female ddY mice were used for this study. The mice were kept in a room of constant temperature (25±2°C) and humidity (55±10%) with a 12 h light/dark cycle. The mice were randomly divided into 3 groups and fed diets containing 25% protein (Exp. 1) (Table 1), or 25% amino acid mixture (Exp. 2 and 3) (Tables 1, 2). In Exp. 3 mice were fed 25% AA-SPI, AA-SPI with reduced Gln/Glu (LG-AA-SPI), AA-EW or AA-EW supplemented with Gln/Glu (HG-AA-EW) (Table 3). At the 5th week, mice were divided into 2 sub-groups ; sensitized and non-sensitized. Nitrogen concentrations of dietary proteins were analyzed using the Kjeldahl method. Protein concentrations were calculated by multiplying nitrogen concentrations and protein conversion ratios (17). The other components of the diets were 5% soybean oil, 2% cellulose powder, 5% mineral mixture (Oriental Co., Tokyo, Japan) and 1% vitamin mixture (Oriental Co., Tokyo, Japan). Remaining parts of the diets were carbohydrates (α-cornstarch:granule sucrose = 2:1). The compositions of the amino acid mixtures (Kyowa Hakko Kogyo Co. Tokyo, Japan) were made following the amino acid composition table of the Japan Science and Technology Agency (17), except Glu. The table contains Glu but not Gln, because Gln in protein form becomes Glu during the hydrolysis of the protein during amino acid analysis. However, Gln is immunologically important, therefore, we used both Gln/Glu. The ratios of Gln and Glu in the proteins used in the present study were calculated following the previous studies (18-23). Mice were fed casein, SPI, EW or gluten diet in Exp .1, amino acid mixture diet patterned after casein (AA-casein), SPI (AA-SPI), EW (AA-EW) or gluten (AA-gluten) in Exp. 2, and AA-SPI, LG-AA-SPI, AA-EW or HG-AA-EW diet in Exp. 3. After the experimental diet for 4 weeks, the mice from each dietary group were divided into 2 sub-groups, sensitized (sen-) and non-sensitized (ns-), depending upon whether they were treated with TDI (Wako Chemical Co. Tokyo, Japan) in ethyl acetate (Wako Chemical Co. Tokyo, Japan) (sensitization) or with the vehicle alone (non-sensitized). The mice were housed approximately 5 per cage under controlled environmental conditions (25±1°C, 55±10% relative humidity, 0800-2000 h lighting period). They were weighed weekly. This study was approved by the Ethical Committee of Tokushima University for Animal Studies.

Sensitization and provocation
Sensitization was done by the method of Tanaka et al. (15) with slight modification. The experimental design for TDI treatment of the study is shown in Fig. 1. The mice were sensitized by dropping 2 μl of 5% TDI dissolved in ethyl acetate in Exp. 1and 1% TDI in Exp. 2 and 3 into the nostrils under slight ether anesthesia for 5 consecutive days. This was repeated following a rest of 9 days. Non-sensitized mice were similarly treated with the vehicle. We used different concentrations of TDI in 3 experiments because mice became very weak after sensitization in Exp. 1, therefore, we reduced the TDI concentration to 1% in Exp. 2 and Exp. 3. The mice were again allowed 9 days rest and then all the mice were provoked with the same volume and concentration of TDI as the sensitization without anesthesia. Nasal responses of itching, water rhinorrhea and snorting were scored for 10 minutes by the method of Irifune et al. (24) (Table 4). Hair loss was scored by observing the extent of hair loss from the snout on the day of provocation (Table4).

Serum IgE measurement
Two weeks (Exp. 1 and 2) or 1 week (Exp. 3) after the provocation with TDI, mice were anesthetized by intraperitoneal administration of pentobarbital (0.1 g/kg body weight) and killed. Serum was removed from collected blood and immediately stored at -40°C until use. Total IgE in serum was determined by a sensitive enzyme-liked immunosorbent assay (ELISA) using 96-well microtitre plates. Each well was coated with anti-mouse IgE (Yamasa Syoyu Co., Chiba, Japan) (1 μg/ml) and incubated overnight at 4°C. The wells were washed 5 times with 25 mM Tris-HCl, pH 7.4, containing 0.14 M NaCl, 5 mM KCl, 0.1% NaN3 and 0.5% Tween 20 (TBS-T). Then the wells were blocked with 3% bovine serum albumin for1 hour at room temperature. After washing 5 times, diluted mouse serum samples or purified anti-DNP-mouse IgE (Yamasa Syoyu Co., Chiba, Japan) (0.025-1 μg/ml) as standards were added to the wells. The standards and serum samples were diluted with 20 mM Tris-HCl, pH 7.4, containing 10 μM phenylmethyl sulphonyl fluoride. The wells were washed 5 times and incubated with biotinylated anti-mouse IgE (1 μg/ml) (Yamasa Syoyu Co., Chiba, Japan) for 1 hour at room temperature. The wells were washed with TBS-T and incubated with avidin-biotin complex solution (Vectastain ABC Elite Kit, Vector Laboratories Burlingame, CA, USA.) for 1 hour at room temperature. Then, after washing five times with TBS-T, paranitrophenyl phosphate solution was added to the wells for color development. After 5 minutes, the color development was terminated by adding150 mM ethylene diamine tetra-acetic acid. The absorbance at 415 nm was read with an automatic ELISA reader.

Histology of nasal tissue
Samples of nasal tissue were removed for histological examination. The tissues were fixed in 10% phosphate buffered formalin at room temperature overnight. The tissues were sliced into 4-6 mm pieces, dehydrated in ethanol, embedded in paraffin wax, sectioned and stained with hematoxylin and eosin (H&E). For the examination of mast cells, the tissues were stained with toluidine blue solution. The sections were examined and photographed using a microscope (Olympus Kogyo Co., Tokyo, Japan).

Statistical analysis
Values are shown as means±SE. The statistical differences were assessed by Duncan's multiple range test. The values with different superscript letters indicate significant difference (p<0.05).

RESULTS
Changes in body weight in Exp. 1
The body weights of the mice were not different among the groups before the first sensitization (at 5th week) (Table 5). After the first sensitization (at 5th week), the sen-EW and sen-Casein groups lost weight, but the sen-SPI and sen-Gluten groups did not. The sen-Casein group recovered body weight after 9 weeks but the sen-EW did not.

Nasal responses to provocation in Exp. 1
The behavior score, measured as a total of nasal responses of itching, watery rhinorrhea and snort during 10 minutes after the provocation by TDI and degree of hair loss are shown in Fig. 2. Generally, the sensitized groups showed higher total scores compared with non-sensitized groups. Significant differences between non-sensitized and sensitized groups were observed in casein, SPI and EW diet groups (p<0.05). The sen-EW group had a significantly higher total score than the other sensitized groups except sen-Casein group (p<0.05).

Total serum IgE concentrations in Exp. 1
Fig. 3 shows findings of serum concentrations of IgE. The concentrations of sensitized groups tended to be higher than those of the non-sensitized groups without significant differences (p>0.05) except in the casein and EW groups (p<0.05). The sen-EW group showed the highest concentration of IgE (p<0.05). There were no significant differences among the non-sensitized groups (p>0.05).

Changes in body weight in Exp. 2
Changes in body weight were not significantly different among all groups (p>0.05), however the sen-AA-EW group lost weight after the 9th week (p<0.05) (Table6).

Nasal responses to provocation in Exp. 2
Fig. 4 shows the total score of nasal responses to provocation in Exp 2. Total score of the sen-AA-EW group was higher than the scores of the other groups (p<0.05).

Serum IgE concentrations in Exp. 2
Fig. 5 shows the serum concentrations of IgE. The sen-AA-EW group showed higher IgE concentration than the other groups (p<0.05). Differences in the other groups were not observed (p>0.05).

Changes in body weight in Exp. 3
Before the first sensitization (5th week), the body weights of the mice were not different among the groups (Table 7). After sensitization, the sen-AA-EW and sen-HG-AA-EW groups lost weight significantly, however, the sen-AA-SPI and sen-LG-AA-SPI did not. After the provocation at the 9th week, all the sensitized groups lost weight. The loss was greatest in the sen-AA-EW and sen-HG-AA-EW groups.

Nasal responses to provocation in Exp. 3
Fig. 6 shows the total score of nasal responses to provocation in Exp. 3. The total score of the sen-AA-EW group was significantly higher than those of the other groups (p<0.05). The scores of the sen-AA-SPI and sen-LG-AA-SPI groups tended to be lower than those of sen-HG-AA-EW group, however, the difference was not significant (p>0.05).

Serum IgE concentrations in Exp. 3
Fig. 7 shows the serum IgE concentrations. The sen-AA-EW group showed significantly higher values than the other groups (p<0.05). The concentration of the sen-HG-AA-EW group was not different from the concentrations of the sen-AA-SPI and sen-LG-AA-SPI groups (p>0.05).

Pathologic findings
Fig. 8 shows typical examples of the microscopic views of nasal membrane in the sensitized and non-sensitized mice.
Fig. 9 shows typical examples of the mast cells of nasal tissue in the sensitized and non-sensitized mice.

DISCUSSION
We studied whether different amino acid mixtures have different effects on nasal allergy induced by TDI and found that mice fed AA-EW had low body weight (Table 6), high nasal scores (Fig. 4) and high serum IgE concentrations (Fig. 5). The findings were similar to those of Exp. 1 in which various proteins were compared. These findings suggest that amino acid mixtures composed of EW, easily induce nasal allergy by TDI sensitization compared with amino acid mixtures, composed of other proteins. The present findings support the hypothesis that some amino acid mixtures may easily induce allergy and hypersensitivity. This finding must be important for the control of allergy and immune hypersensitivity.
Some mechanisms can be suggested to explain the above findings. One is the high intake of high quality proteins. Poor protein nutrition reduces immunity and then resistance against infection (25, 26), indicating that good protein nutrition enhances immunity. We found that nutrients which enhance immunity also easily induced hypersensitivity by the over intake as discussed in the introduction of this paper (1-10). Protein quality of EW is the highest of all food types or groups. For example, the biological values reported by Food and Agriculture Organization and World Health Organization are 94 for EW, 82 for casein, 65 for SPI and 48 for gluten (27). Therefore, EW may be prone to induce hypersensitivity because of its high protein quality. This hypothesis can easily explain why the incidence of allergy is very high in Japan and other developed countries. From the greatly increased consumption in protein rich foods during the last half a century in Japan, protein intake has increased greatly as shown in the report of the annual nutrition survey by the Ministry of Health and Welfare (28). More than half of the protein is supplied by animal foods, indicating that the quality and quantity of amino acids derived from the ingested proteins are very high in Japan.
Another possible factor of allergy aggravated by EW and AA-EW diets is the specific amino acids. Gln upregulates immunity (9, 10). We have found that Gln supplementation alleviates inflammatory bowel disease induced by TNBS in rats (17), although high supplementation aggravates it (12). Those studies (9-12) indicated that Gln plays an important role in immunity, however, the effect on allergy is not known. The concentrations of Gln were 33.7% in gluten, 10.9% in casein, 8.1% in SPI and 3.7% in EW (Table 2). EW has the lowest concentration of Gln. Therefore, in Exp. 3 we examined whether the low concentration of Gln was a factor in the severe allergy aggravated by EW. We decreased Gln in AA-SPI and increased Gln in AA-EW. Gln supplementation to AA-EW (HG-AA-EW) was effective in alleviating the nasal symptoms and to reducing the IgE level, but was not effective in preventing body weight loss. However, Gln reduction in the SPI groups showed no effects. The findings of Exp. 3 suggest that low Gln in EW is one of the factors of severe allergy in mice fed amino acid mixtures patterned after egg white protein. However, for a definitive clarification more studies are required. Further studies are required as the effects of other amino acids, for example, cystine which is very high in EW.
From the present study we can conclude that amino acid mixtures affect allergy. This suggests that for the control of allergy it is necessary to pay more attention to the pattern and the concentration of amino acids in diets.

ACKNOWLEDGEMENT
We thank FANCL Co. for their financial support to this research and Kyowa Hakko Kogyo Co. for their donation of amino acids.

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Received for publication May 22, 2000;accepted June 19, 2000.

Address correspondence and reprint requests to Tzu-Hsiu Chen, MSc, Department of Nutrition, The University of Tokushima School of Medicine, Kuramoto-cho, Tokushima 770-8503, Japan and Fax:+81-88-633-9427.